Application

NovaTaq Hot Start DNA Polymerase has been used in polymerase chain reaction (PCR) amplification. It is suitable for use in Hot Start routine PCR.

Components

250 U or 5 × 250 UNovaTaq Hot Start DNA Polymerase (5 U/µl)r> 1.5 ml or 5 × 1.5 ml10X NovaTaq Hot Start Buffer 1.5 ml or 5 × 1.5 ml25 mM MgCl₂

Features and Benefits

Higher PCR specificity and yield Improved low-copy target amplification Ambient temperature setup compatible with automation Target amplification of up to 5 kbp Ideal for quantitative and high-throughput PCR applications

General description

NovaTaq Hot Start DNA Polymerase is a chemically modified Taq DNA Polymerase, which is inactive at room temperature. This polymerase is a derived recombinant form of Thermus aquaticus DNA polymerase. The enzyme exhibits a 5′→3′ DNA polymerase activity and lacks 3′→5′ exonuclease activity. The enzyme provides improved specificity when compared to standard Taq polymerase and can eliminate the presence of nonspecific amplification such as primer-dimers and mis-primed products. The enzyme must be activated by heat treatment (7–10 min at 95°C), after which thermal cycling can proceed. NovaTaq Hot Start DNA Polymerase generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen^® Perfectly Blunt^®, Acceptor, and LIC Vector Kits. NovaTaq Hot Start DNA Polymerase is a component of NovaTaq Hot Start Master Mix Kit.Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coliConcentration: 5.0 U/μlEndonuclease: None detectedExonuclease: None detectedAmplification efficiency: Functional PCRStorage: –20°

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1250, 250 u in Glass bottle

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [A-³²P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)